TRIB3 Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > TRIB3 Knockout cell line (Hep G2)

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TRIB3 Knockout cell line (Hep G2) Order

TRIB3 Knockout cell line (Hep G2)
Catalog#
YKO-HT2915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRIB3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRIB3 Knockout cell line (Hep G2)
Species
Human
Gene Name
TRIB3
Gene ID
57761
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript TRIB3-201)
Validation: PCR+Sanger Sequencing
TRIB3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRIB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRIB3 gene knockout TRIB3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TRIB3 Knockout cell line (Hep G2)
TRIB3 Knockout cell line (Hep G2)
Catalog#
YKO-HT2915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TRIB3 Knockout cell line (THP-1)

    TRIB3 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HT0465
    YKO-HT0465(THP-1)
  • TRIB3 Knockout cell line(U-87MG)

    TRIB3 Knockout cell line(U-87MG)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-H510
    YKO-H510(U-87 MG)
  • TRIB3 Knockout cell line (HeLa)

    TRIB3 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HS13500
    YKO-HS13500(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TRIB3 Knockout cell line (THP-1)

    TRIB3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HT0465
      inquiry   More details
  • TRIB3 Knockout cell line(U-87MG)

    TRIB3 Knockout cell line(U-87MG)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-H510
      inquiry   More details
  • TRIB3 Knockout cell line (HeLa)

    TRIB3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HS13500
      inquiry   More details

TRIB3 Knockout cell line (Hep G2) Order

TRIB3 Knockout cell line (Hep G2)
Catalog#
YKO-HT2915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRIB3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRIB3 Knockout cell line (Hep G2)
Species
Human
Gene Name
TRIB3
Gene ID
57761
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript TRIB3-201)
Validation: PCR+Sanger Sequencing
TRIB3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRIB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRIB3 gene knockout TRIB3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TRIB3 Knockout cell line (Hep G2)
TRIB3 Knockout cell line (Hep G2)
Catalog#
YKO-HT2915
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TRIB3 Knockout cell line (THP-1)

    TRIB3 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HT0465
    YKO-HT0465(THP-1)
  • TRIB3 Knockout cell line(U-87MG)

    TRIB3 Knockout cell line(U-87MG)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-H510
    YKO-H510(U-87 MG)
  • TRIB3 Knockout cell line (HeLa)

    TRIB3 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HS13500
    YKO-HS13500(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • TRIB3 Knockout cell line (THP-1)

    TRIB3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HT0465
      inquiry   More details
  • TRIB3 Knockout cell line(U-87MG)

    TRIB3 Knockout cell line(U-87MG)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-H510
      inquiry   More details
  • TRIB3 Knockout cell line (HeLa)

    TRIB3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TRIB3
    Gene ID: 57761
    Size: 1*10^6
    Catalog#: YKO-HS13500
      inquiry   More details

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